Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)

摘要: We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From (AG)-enriched library, we identified 66 unique sequences which primers could be designed. Twenty-two reliably amplified a clear single band expected size, and were scoreable polymorphic. For these loci, genotyped monoecious dioecious population, yielding four 14 alleles per locus. Three exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight also in other species. These will useful compare genetic structure among populations S. latifolia.