Characterization of Lactococcus lactis isolates from bovine mastitis

摘要: Lactococcus lactis is a widely used mesophilic dairy starter and has been included in the Qualified Presumption of Safety (QPS) list European Food Authority. However, it increasingly found as cause human or animal infections, such bovine mastitis, probably due to improvement identification infective microorganisms. Since there are some grounds suspect that at least certain variants L. may diseases, important properly identify differences between strains associated with infections safe strains. Bovine mastitis isolates were genotypically characterized clustered by 16S rRNA gene sequence RAPD-PCR fingerprint patterns, phenotypically their tolerance different stress conditions typically intestinal tract mammals, carbohydrate- antibiotic resistance profile, well vitro adhesion capacity udder epithelial cells. Genotypically, no clear phenotypic distinctions typical strains, former showing an increased temperature, lysozyme, bile salts, pH antibiotics, improved carbohydrate fermentation capacity, Although these might not be considered actual virulence factors, they improve ability strain survive body homeothermic animals and, interestingly, also properties potential probiotic