PCR-based detection of DNA from the human pathogen Blastomyces dermatitidis from natural soil samples.
作者： J. W. Burgess , W. R. Schwan , T. J. Volk
DOI: 10.1080/13693780600954749
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摘要: Blastomyces dermatitidis is the dimorphic fungal agent of blastomycosis, a disease that primarily affects humans and dogs. The clinical appearance this mycosis well characterized, but there still little known about its environmental niche, having been isolated from nature only 21 times. We have developed PCR-based assay to detect B. soil samples using primers specific portion promoter region BAD1 virulence gene. An internal standard control, pTJV2, was constructed validate results samples. Amplification control indicated adequate removal ambient inhibitors. PCR detection limits for plasmid genomic DNA were 0.1 500 femtograms, respectively. No cross-reactivity observed against bacteria, actinomycetes, 13 other fungi genetically related or found in same geographic areas. In spiked samples, method sensitive 304 copies pTJV2 8,450 live B...
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