Molecular Characterization of Three Plasmids From Bifidobacterium Longum
作者： Nathalie Corneau , Éric Émond , Gisèle LaPointe
DOI: 10.1016/J.PLASMID.2003.12.003
关键词:
摘要: The complete nucleotide sequences for pNAC1 (3538bp) from strain RW048 as well pNAC2 (3684bp) and pNAC3 (10,224bp) RW041 of Bifidobacterium longum were determined. largest ORF (repB) encodes a putative protein similar to those involved in rolling-circle (RC) replication mechanism, which was confirmed by demonstration single-strand intermediates the host cell. RepB gene product is most pDOJH10L pKJ36. A second (mob) mobilization proteins conjugation. Plasmid bifidobacterial plasmid be sequenced date. Of eight products coded pNAC3, one (RepB), another (Orf2) transfer (Tra). Bifidobacterial plasmids divided into five groups based on Rep amino acid sequence homology results suggest new family B. longum.
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