Endurance-Type Exercise Increases Bulk and Individual Mitochondrial Protein Synthesis Rates in Rats

摘要: Physical activity increases muscle protein synthesis rates. However, the impact of exercise on coordinated up- and/or downregulation individual rates in skeletal tissue remains unclear. The authors assessed mixed muscle, myofibrillar, and mitochondrial as well vivo rats. Adult Lewis rats either remained sedentary (n = 3) or had access to a running wheel for last 2 weeks 3-week experimental period. Deuterated water was injected subsequently administered drinking over Blood soleus were collected used assess bulk using gas chromatography-mass spectrometry liquid (i.e., dynamic proteomic profiling). Wheel resulted greater myofibrillar (3.94 ± 0.26 vs. 3.03 0.15%/day; p < .01) (4.64 0.24 3.97 0.26%/day; .05), but not (2.64 0.96 2.38 0.62%/day; .71) rates, when compared with condition. Exercise impacted 80 proteins, difference from condition ranging between -64% +420%. Significantly detected F1-ATP synthase, ATP synthase subunit alpha, hemoglobin, myosin light chain-6, synaptopodin-2 (p .05). adaptive response endurance-type involves upregulation it is highly reflected by various proteins across different subcellular fractions.