The association between methylation patterns of DNAH17 and clinicopathological factors in hepatocellular carcinoma.

摘要: BACKGROUND Hepatocellular carcinoma (HCC) is a malignancy with poor prognosis. Complex genetic and epigenetic alterations are the two primary causes of HCC. The aim study was mainly to explore correlation between methylation status DNAH17 METHODS We evaluated levels in 163 HCC samples their paired normal tissue using Sequenom EpiTYPER assays performed TaqMan copy number assay assess samples. RESULTS mean were significantly decreased tumor tissues compared both selected regions (amplicon 1:58.7% vs 84.5%, P < 0.0001; amplicon 2:69.9% P = 0.0060). Contrarily，both RNA-seq immunohistochemistry indicated expression increased (P < 0.05). DNMT inhibitor decitabine treatment could increase cell lines. gene amplification always companied hypomethylation status. Moreover, associated several clinical characteristics, such as male patients, higher AFP values, age onset, fibrous capsules, necrosis, liver cirrhosis, thrombus Receiver operator characteristic (ROC) curve analysis demonstrated efficiently predict existence capsule (AUC = 0.695) (AUC = 0.806). CONCLUSIONS These findings suggested that aberrant comprehensive clinicopathological factors be promising biomarker for thrombosis patients.