Mechanism of rhein-induced apoptosis in rat primary hepatocytes: beneficial effect of cyclosporine A.

摘要: Past observational and toxicity studies have established an association between the deaths of children consumption Cassia occidentalis (CO) seeds. We recently reported chemical evidence this following identification toxic anthraquinones (AQs), viz. aloe-emodin, chrysophanol, emodin, physcion, rhein, in CO seeds (Panigrahi, G. K. et al. (2015), Chem. Res. Toxicol. DOI: 10.1021/acs.chemrestox.5b00056). Of these five AQs, earlier shown rhein to be most cytotoxic AQ hepatocytes. Therefore, present study was designed investigate effect on rat primary Results indicated that (50 μM) causes apoptosis hepatocytes by generating reactive oxygen species (ROS), increasing intracellular Ca2+, decreasing mitochondrial membrane potential, depleting glutathione content. At molecular level, rhein-induced DNA damage results overexpression γ-H2AX protein (2.5-fold), thereby causing enhanc...