BioPEGylation of polyhydroxybutyrate promotes nerve cell health and migration.
作者： Rodman T. H. Chan , Robert A. Russell , Helder Marçal , Terry H. Lee , Peter J. Holden
DOI: 10.1021/BM401572A
关键词:
摘要: This study reports on the superior suitability of Polyhydroxybutyrate-polyethylene glycol hybrid polymers biosynthesised by Cupriavidus necator over PHB as biomaterials for tissue engineering. Incorporation PEG106 (DEG) during biosynthesis reduced crystallinity, molecular weight, and hydrophobicity while improving mechanical properties. In vitro olfactory ensheathing cell (OEC) proliferation was enhanced cultivation PHB-b-DEG films. Cultivation films showed no cytotoxic responses viability membrane integrity sustained. promoted OECs entering into DNA replication (S) phase mitotic (G2-M) growth cycle apoptosis low. also confirmed an association between level neurite-outgrowth inhibitory protein (Nogo) receptor pair Ig-like B (PirB) expression proliferation, both being down-regulated in cells grown when compared with asynchronous growth. Thus, DEG-terminated PHB-based have great potential biological scaffolds supporting nerve repair.
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