Synthesis and application of a ratiometric probe for hydrogen peroxide

摘要: Abstract Molecular imaging of biological analytes provides detailed insights into signaling processes. Ratiometric probes are particularly attractive due to the ability quantify analyte production. However, design strategies for ratiometric can be hindered by spectral overlap product and reactant species. In this chapter, we provide protocols synthesis application RF620, a probe H2O2 displaying dramatic changes in both excitation emission wavelengths, designed using an approach term chemoselective alteration fluorophore scaffolds. The contains functional group within fluorescent xanthene scaffold, resulting situ new upon reaction with H2O2. Under physiological conditions, RF620 exhibits far-red near-infrared emission, H2O2, is chemically converted tetramethylrhodamine, producing significant (~ 66 nm) blue-shift emission. used ratiometric, molecular endogenous production living cells.