Isolation of bovine eosinophils and characterization of their leukotriene formation
作者： J. Freiburghaus , A. Jörg
DOI: 10.1007/BF02003216
关键词:
摘要: A method for the isolation of eosinophils from bovine blood is described. Eosinophils were prepurified in a simple centrifugation step and subsequently separated on Percoll gradient into two populations with densities 1.088 1095 g/cm3, respectively, purity greater than 90%. No apparent differences could be observed electron micrographs most granules appeared homogenous. minor fraction to have lamellated crystalline core structure. generate leukotriene B4 its stereoisomers but not sulfidopeptide leukotrienes (slow-reacting substance) when stimulated by Ca-ionophore A23187.
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