Interaction of the EWS NH2 Terminus with BARD1 Links the Ewing’s Sarcoma Gene to a Common Tumor Suppressor Pathway
作者： Christine Siligan , Dave N. T. Aryee , Robert Petermann , Heinrich Kovar , Johannes A. Schmid
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摘要: In 85% of Ewing family tumors, the NH 2 terminus EWS is fused to DNA-binding domain FLI1, an ets transcription factor. The resulting chimeric protein a strong transcriptional activator with transforming activity. We report that and EWS-FLI1 interact via their common COOH BARD1, putative tumor suppressor, in vitro vivo . Because BARD1 associates its -terminal RING breast cancer susceptibility gene BRCA1 provides platform for interactions proteins involved DNA repair checkpoint control, our results provide link between Ewing’s sarcoma product genome surveillance complex.
aacrjournals.org参考文章(26)
Angus Chen, Frida E. Kleiman, James L. Manley, Toru Ouchi, Zhen-Qiang Pan, Autoubiquitination of the BRCA1*BARD1 RING ubiquitin ligase. Journal of Biological Chemistry. ,vol. 277, pp. 22085- 22092 ,(2002) , 10.1074/JBC.M201252200
Michael A. Teitell, Scott M. Welford, Christopher T. Denny, Afsane Arvand, The COOH-terminal domain of FLI-1 is necessary for full tumorigenesis and transcriptional modulation by EWS/FLI-1. Cancer Research. ,vol. 61, pp. 5311- 5317 ,(2001)
Dave N. T. Aryee, Claudia Hattinger, Helmut Gadner, Andreas Zoubek, Gunhild Jug, Heinrich Kovar, Laura Spahn, Peter F. Ambros, The EWS Protein Is Dispensable for Ewing Tumor Growth Cancer Research. ,vol. 61, pp. 5992- 5997 ,(2001)
Ki-Baik Hahm, Keuna Cho, Cecile Lee, Young-Hyuck Im, Jay Chang, Shin-Geon Choi, Poul H.B. Sorensen, Carol J. Thiele, Seong-Jin Kim, Repression of the gene encoding the TGF-beta type II receptor is a major target of the EWS-FLI1 oncoprotein. Nature Genetics. ,vol. 23, pp. 222- 227 ,(1999) , 10.1038/13854
Johannes A. Schmid, Petra Scholze, Oliver Kudlacek, Michael Freissmuth, Ernst A. Singer, Harald H. Sitte, Oligomerization of the Human Serotonin Transporter and of the Rat GABA Transporter 1 Visualized by Fluorescence Resonance Energy Transfer Microscopy in Living Cells Journal of Biological Chemistry. ,vol. 276, pp. 3805- 3810 ,(2001) , 10.1074/JBC.M007357200
Y. Jin, X. L. Xu, M.-C. W. Yang, F. Wei, T.-C. Ayi, A. M. Bowcock, R. Baer, Cell cycle-dependent colocalization of BARD1 and BRCA1 proteins in discrete nuclear domains Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 12075- 12080 ,(1997) , 10.1073/PNAS.94.22.12075
Frida E. Kleiman, James L. Manley, The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression. Cell. ,vol. 104, pp. 743- 753 ,(2001) , 10.1016/S0092-8674(01)00270-7
Leeju C Wu, Zhuo Wei Wang, Julia Tsou Tsan, Monique A Spillman, Anne Phung, Xie L Xu, Meng-Chun W Yang, Larn-Yuan Hwang, Anne M Bowcock, Richard Baer, None, Identification of a RING protein that can interact in vivo with the BRCA1 gene product. Nature Genetics. ,vol. 14, pp. 430- 440 ,(1996) , 10.1038/NG1296-430
Scott M. Welford, Stephen P. Hebert, Benjamin Deneen, Afsane Arvand, Christopher T. Denny, DNA Binding Domain-independent Pathways Are Involved in EWS/FLI1-mediated Oncogenesis Journal of Biological Chemistry. ,vol. 276, pp. 41977- 41984 ,(2001) , 10.1074/JBC.M106757200
Irmgard Irminger-Finger, Wai-Choi Leung, Jian Li, Michel Dubois-Dauphin, Jean Harb, Anis Feki, Charles Edward Jefford, Jesus V Soriano, Marisa Jaconi, Roberto Montesano, Karl-Heinz Krause, None, Identification of BARD1 as Mediator between Proapoptotic Stress and p53-Dependent Apoptosis Molecular Cell. ,vol. 8, pp. 1255- 1266 ,(2001) , 10.1016/S1097-2765(01)00406-3