Hepatitis C virus protein expression causes calcium‐mediated mitochondrial bioenergetic dysfunction and nitro‐oxidative stress

摘要: Hepatitis C virus (HCV) infection induces a state of oxidative stress that is more pronounced than in many other inflammatory diseases. In this study we used well-characterized cell lines inducibly expressing the entire HCV open-reading frame to investigate impact viral protein expression on bioenergetics. It was shown has profound effect metabolism, with specific inhibition complex I activity, depression mitochondrial membrane potential and phosphorylation coupling efficiency, increased production reactive oxygen nitrogen species, as well loss Pasteur effect. Importantly, all these effects were causally related calcium overload, uptake completely reversed observed bioenergetic alterations. Conclusion: Expression proteins causes deregulation homeostasis. This event occurs upstream further dysfunction, leading alterations balance nitro-oxidative stress. These observations provide new insights into pathogenesis hepatitis may offer opportunities for therapeutic intervention. (HEPATOLOGY 2007.)