High resolution localization of angiotensin II receptors in rat renal medulla

摘要: High resolution localization of angiotensin II receptors in rat renal medulla. The cellular (Ang II) the inner stripe outer medulla kidney was investigated by using high light and electron microscopic autoradiography. Fresh tissue blocks from were incubated with 125 I-[Sar 1 Ile 8 ] Ang prepared for At level, found to penetrate into bind specifically sites outlining tubules vasa recta bundles. Electron autoradiography revealed that silver grains detected over interstitial cells located between components bundles, but no overlying thin descending or thick ascending limbs loop Henle, collecting ducts, recta, other blood vessels. These contained abundant endoplasmic reticulum, microfilaments, occasional lipid droplets extensive cytoplasmic processes which closely related basement membranes loops Henle. therefore resemble type cells. Since binding are absent medulla, labelled this technique must be a subset cells, distinct typical lipid-laden most findings demonstrate primary density