Parameters and determinants of responses to selection in antibody libraries

摘要: Antibody repertoires contain binders to nearly any target antigen. The sequences of these antibodies differ mostly at few sites located on the surface a scaffold that itself consists much less varied amino acids. What is impact this response selection repertoire? To gauge impact, we carried out quantitative phage display experiments with three antibody libraries based distinct scaffolds harboring same diversity randomized sites, which selected for binding four arbitrary targets. We first show an library captured by simple and measurable parameter direct physical information-theoretic interpretations. Second, identify major determinant encoded in scaffold, its degree evolutionary maturation. Antibodies undergo accelerated process, called affinity maturation, improve their given antigen as part adaptive immune response. find built around such maturated have lower other targets than naive germline origin. Our results are step towards quantifying controlling potential biomolecules.