Identification of 69-kd and 100-kd forms of 2-5A synthetase in interferon-treated human cells by specific monoclonal antibodies.

摘要: Abstract Recently, the existence of 40-kd and 46-kd 2-5A synthetases in interferon-treated cells has been confirmed by cloning characterization cDNA corresponding to these small size enzymes. By use specific monoclonal antibodies, we describe here two forms high mol. wt 69 100 kd human cells. The antibodies immunoprecipitate either a 69- or 100-kd synthetase. These purified immune complex preparations are active, i.e. addition poly(I).poly(C) ATP results synthesis 2-5A. Both composed several subspecies with similar isoelectric points range 7-8 but have different subcellular localizations: synthetase is recovered from microsomal pellet whereas 69-kd found be associated cell membranes as well pellet. Different types express both were also identified electrophoretic transfer immunoblot analysis using rabbit polyclonal against synthetic peptide common on 46- synthetases. indicate that large isozymes share sequence.