A sensitive and specific assay for histidine decarboxylase activity
作者： Robert J. Levine , Doris E. Watts
DOI: 10.1016/0006-2952(66)90161-4
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摘要: Abstract A method for assay of histidine decarboxylase activity is described; it based upon using as substrate 14C-histidine labeled in the carboxyl carbon and trapping hydroxide hyamine 14CO2 evolved during incubation. This procedure may be applied to individual tissue specimens after gel-filtration chromatography homogenates. For illustrative purposes assays gastric homogenates are presented. Among advantages offered by this technique over those previously reported simplicity, specificity, sensitivity, capability expressing enzyme terms total histamine formed per unit original tissue. Histidine decarboxylases from fetal rat were found stereospecific l -histidine not inhibited high concentrations histamine. Data presented that suggest presence an unidentified soluble inhibitor
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