Specific Hydrolysis of the Cohesive Ends of Bacteriophage λ-Deoxyribonucleic Acid by Micrococcus luteus Ultraviolet Exonuclease

摘要: Abstract Conditions have been established under which Micrococcus luteus ultraviolet exonuclease cleaves off part of the 12-nucleotide-long single-stranded cohesive ends λDNA, leaving either 3 or 4 nucleotides on each end, as determined by repair incorporation radioactive into remaining DNA polymerase I. This finding is supported results from nearest neighbor and 3' end analyses indicate that cleavage almost, but not completely, synchronous. The exonuclease-digested λDNA can be further degraded Escherichia coli III to increase length ends. After synthesis, nucleotide sequence newly exposed segments without having repeat analysis entire digested this way gave expected