Segmentation propagation using a 3D embryo atlas for high-throughput MRI phenotyping: Comparison and validation with manual segmentation

摘要: Effective methods for high-throughput screening and morphometric analysis are crucial phenotyping the increasing number of mouse mutants that being generated. Automated segmentation propagation embryo is an emerging application enables noninvasive rapid quantification substructure volumetric data analysis. We present a study to assess validate accuracy brain kidney volumes generated via in ex vivo MRI atlas comprising three different groups against current ‘‘gold standard’’—manual segmentation. Morphometric assessment showed good agreement between automatically manually segmented volumes, demonstrating it possible population embryos using with same variation as manual As part this study, we have made our average freely available community use studies. These datasets automated analyses will be essential meeting challenge high-throughput, phenotyping. Magn Reson Med 000:000– 000, 2012. V C 2012 Wiley Periodicals, Inc. The established model system studying characterising role mammalian gene function human diseases. In particular, used investigate genetic basis development congenital abnormalities. Completion genome sequence (1) tools manipulation (2) led worldwide initiative generate large numbers mice knockouts each � 25,000 genes