In vitro and in vivo anticancer activity of posterior salivary gland toxin from the cuttlefish Sepia pharaonis, Ehrenberg (1831)

摘要: Abstract Posterior salivary gland (PSG) toxins are high molecular weight secreted by cephalopods and gastropods which possess immense potentials in biomedical applications. In the present study, of PSG toxin from cuttlefish, S. pharaonis was determined in vitro in vivo. The cytostatic lymphocyte migration inhibition assay. (50 μg/ml) effectively inhibited lymphocytes across agarose gel matrix under presence lipopolysaccharide mitogen. cytotoxicity against cancer cell lines using MTT exhibited highest MCF-7 breast cells (IC50-10.64 μM) followed KB, HeLa A549 cells. also proportional release LDH leakage mitochondrial damage with an IC50-13.85 μM cells. Flow cytometry analysis revealed that induced apoptosis MCF-7 cells cycle arrest at G0/G1 phase. (80 mg/kg b.w.) pronounced reduction (29%) tumor growth experimentally carcinoma female Balb/C mice, Hematological illustrated restoration blood biochemical parameters mice tumor. Histopathology studies restitution morphological features mammary vital organs treated without any observed toxicity adverse effects. further commendable prevention metastasis into immediate viz lungs, thus functioning as anti-metastatic agent. results study showed promise a potential peptide based anticancer agent, future.