Membrane Trafficking and Mechanics of the Golgi Apparatus: An in Cellulo Study by Optical Micromanipulation

摘要: Membrane transport is based on the formation of tubulo-vesicular intermediates traveling from one compartment cell to another along cytoskeletal tracks. In vitro studies have shown that physical parameters, such as membrane curvature, tension and composition, influence budding fission intermediates. A recent study in cells has highlighted central role actin cytoskeleton Rab6-positive Golgi apparatus (Miserey-Lenkei et al. 2010). Here we investigate a mechanical stress intracellular cellulo. We focus mechanics membranes apparatus. Using confocal microscopy, visualize deformation applied by an internalized microsphere trapped optical tweezers, simultaneously measure corresponding forces. Our results show force necessary deform drops when depolymerized, suggesting strongly contributes local rigidity also long-range effect induces sharp decrease vesicles