Directed enzyme evolution : screening and selection methods

摘要: Part I. Genetic Selections Complementation Protocols Jessica L. Sneeden and Lawrence A. Loeb Use of Pol I-Deficient E. coli for Functional DNA Polymerase Manel Camps Selection Novel Eukaryotic Polymerases by Mutagenesis Yeast Ranga N. Venkatesan Autogene Jijumon Chelliserrykattil Andrew D. Ellington Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase Volker Sieber Proside: A Phage-Based Method Selecting Thermostable Andreas Martin, Franz X. Schmid, Minimization Random Fragmentation Gary W. Rudgers Timothy Palzkill II. Screens Enzymes Evaluating a Screen Analysis Mutant Libraries Oriana Salazar Lianhong Sun Screening in Saccharomyces cerevisiae Thomas Bulter, Sieber, Miguel Alcalde Solid-Phase Using Digital Image Alexander V. Tobias John M. Joern Thermostability Patrick C. Cirino Radu Georgescu High-Throughput a-Amylase Increased Activity at 129 DegreesC Holger Berk Robert J. Lebbink Carbon Monoxide Binding Assay Cytochromes P450 Christopher R. Otey Aromatic Hydroxylation Colorimetric Aliphatic Cytochrome p-Nitrophenyl-Substituted Alkanes Edgardo T. Farinas Based on NAD(P)H Depletion Anton Glieder Peter Meinhold Tetramethylbenzidine (TMB) Peroxidases Screenfor Oxidases Detection Hydrogen Peroxide Horseradish Peroxidase Makoto Yagasaki Dehydrogenase Generation Kimberly Mayer Assays Laccases Bulter pH Sensing Agar Plate Esterolytic Enzyme Karl Griswold pH-Indicator-Based Hydrolytic Haloalkane Dehalogenase Huimin Zhao a-Hydroxyketones Tetrazolium Salts Michael Breuer Bernhard Hauer Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination Koichiro Murashima Roy H. Doi Strategies Disulfide Isomerase Ronald Lafond, Xiaoming Zhan, George Georgiou An Overview Systems Enantioselective Enzymatic Transformations Manfred Reetz Select ee-Screening Assaying Directed Evolution the Substrate Specificities Site-Specific Recombinase an Aminoacyl-tRNA Synthetase Fluorescence-Activated Cell Sorting (FACS) Stephen Santoro G. Schultz Calmodulin-Tagged Phage Two-Filter Sandwich Identification Activities Christian Heinis, Julian Bertschinger, Dario Neri FACS Specificity Mark Olsen, Jongsik Gam, Brent Iverson, Improving Protein Folding Efficiency GFP Reporter Geoffrey S. Waldo Index