Molecular cloning and expression of the cDNA for a novel A2-adenosine receptor subtype.
作者： J H Stehle , S A Rivkees , J J Lee , D R Weaver , J D Deeds
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摘要: A novel adenosine receptor subtype has been cloned from a rat brain cDNA library using a probe generated by the polymerase chain reaction. The cDNA, designated RFL9, encodes a protein of 332 amino acids. The structure of RFL9 is most similar to that of the recently cloned rat A2-adenosine receptor, with a sequence identity of 73% within the presumed seven transmembrane domains. Expression of RFL9 in COS-6M cells resulted in ligand binding and functional activity characteristics of an adenosine receptor that is coupled …
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