Distinct Roles for ROCK1 and ROCK2 in the Regulation of Keratinocyte Differentiation

摘要: Background: The human epidermis is comprised of several layers specialized epithelial cells called keratinocytes. Normal homoeostasis the requires that balance between keratinocyte proliferation and terminal differentiation be tightly regulated. mammalian serine/threonine kinases (ROCK1 ROCK2) are well-characterised downstream effectors small GTPase RhoA. We have previously demonstrated RhoA/ROCK signalling pathway plays an important role in regulation differentiation. In this paper we addressed question which ROCK isoform was involved Methodology Principal Findings: used RNAi to specifically knockdown ROCK1 or ROCK2 expression cultured depletion results decreased adhesion fibronectin increase Conversely, increased inhibits Conclusion: These data suggest play distinct roles regulating