摘要: The writer has shown,2 by means of experiments made in 1920 at the Marine Biological Laboratory, Woods Hole, Mass., that a given dilution sea-water with tap water which leaves uninseminated egg Echinarachnius parma intact is rapidly injurious to inseminated during its process membrane separation. After separation likewise resistant action dilute sea-water. Certain were also same season learn if it possible procure fertilization and development diluted susceptible. These repeated May June I92I laboratory essentially results. In discover seawater, period separation, fertilize egg, was soon apparent cleavage normal gastrulation are not permits Thus 21 28, I92I, inclusive, eggs from twenty-four females varying dilutions. all cases due precautions taken as bulk eggs, quantity solution, density sperm suspensions used order far conditions be uniform. results obtained 95 per cent. sea-watef (95 parts plus 5 water) dilutions ranging this 80 (80 20 may
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