Neuropeptide Y promotes neurogenesis and protection against methamphetamine-induced toxicity in mouse dentate gyrus-derived neurosphere cultures.

摘要: Methamphetamine (METH) is a psychostimulant drug of abuse that causes severe brain damage. However, the mechanisms responsible for these effects are poorly understood, particularly regarding impact METH on hippocampal neurogenesis. Moreover, neuropeptide Y (NPY) known to be neuroprotective under several pathological conditions. Here, we investigated effect dentate gyrus (DG) neurogenesis, cell death, proliferation and differentiation, as well role NPY by itself against METH-induced toxicity. DG-derived neurosphere cultures were used evaluate or neuronal differentiation. its receptors (Y(1), Y(2) Y(5)) was conditions DG death. death both apoptosis necrosis at concentrations above 10 nM, without affecting proliferation. Furthermore, non-toxic concentration (1 nM), decreased NPY's protective mainly due reduction glutamate release, it also increased differentiation via Y(1) receptors. In addition, while activation able prevent subtype alone blocking decrease in induced drug. Taken together, negatively affects viability revealed promising tool deleterious