Chromosome 9 deletion in sporadic and familial melanomas in vivo.

摘要: Twenty microsatellite loci on chromosome 9 were analysed for allelic losses in DNAs from 30 uncultured melanomas 25 patients, relative to DNA autologous peripheral blood lymphocytes. All patients constitutionally heterozygous at several loci, and loss of heterozygosity (LOH) affecting 9p was observed melanoma 18 individuals (72%). Observations identical alleles different metastatic lesions the same LOH a vertical growth phase primary melanoma, consistent with previous reports deletion early development. data suggested entire copies 11 cases, terminal all or portion six cases. A somatic interstitial between D9S162 D9S169 seen familial melanoma. This 21 cM deleted region corresponded previously reported positions homozygous deletions cell lines, susceptibility locus (MLM). As 16 cases present study no family history it is likely that MLM plays role development most sporadic melanomas.