Mammalian Ku86 protein prevents telomeric fusions independently of the length of TTAGGG repeats and the G‐strand overhang
作者： Enrique Samper , Fermín A Goytisolo , Predrag Slijepcevic , Paul PW van Buul , María A Blasco
DOI: 10.1093/EMBO-REPORTS/KVD051
关键词:
摘要: Ku86 together with Ku70, DNA-PKcs, XRCC4 and DNA ligase IV forms a complex involved in repairing double-strand breaks (DSB) mammals. Yeast Ku has an essential role at the telomere; particular, deficiency leads to telomere shortening, loss of clustering, telomeric silencing deregulation G-overhang. In mammals, proteins associate repeats; however, possible regulating length not yet been addressed. We have measured different cell types from wild-type Ku86-deficient mice. contrast yeast, does result shortening or G-strand overhang. Interestingly, Ku86–/– cells show fusions long telomeres (>81 kb) fusion point. These results indicate that mammalian plays fundamental by preventing independently TTAGGG repeats integrity
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Gloria C. Li, Honghai Ouyang, Xiaoling Li, Hatsumi Nagasawa, John B. Little, David J. Chen, C.Clifton Ling, Zvi Fuks, Carlos Cordon-Cardo, Ku70: A Candidate Tumor Suppressor Gene for Murine T Cell Lymphoma Molecular Cell. ,vol. 2, pp. 1- 8 ,(1998) , 10.1016/S1097-2765(00)80108-2
C.M. Counter, A.A. Avilion, C.E. LeFeuvre, N.G. Stewart, C.W. Greider, C.B. Harley, S. Bacchetti, Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. The EMBO Journal. ,vol. 11, pp. 1921- 1929 ,(1992) , 10.1002/J.1460-2075.1992.TB05245.X
H.-L. Hsu, D. Gilley, E. H. Blackburn, D. J. Chen, Ku is associated with the telomere in mammals Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 12454- 12458 ,(1999) , 10.1073/PNAS.96.22.12454
J. M. J. M. Zijlmans, U. M. Martens, S. S. S. Poon, A. K. Raap, H. J. Tanke, R. K. Ward, P. M. Lansdorp, Telomeres in the mouse have large inter-chromosomal variations in the number of t2ag3 repeats Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 7423- 7428 ,(1997) , 10.1073/PNAS.94.14.7423
Kevin D Mills, David A Sinclair, Leonard Guarente, MEC1-Dependent Redistribution Of The SIR3 Silencing Protein from Telomeres to DNA Double Strand Breaks Cell. ,vol. 97, pp. 609- 620 ,(1999) , 10.1016/S0092-8674(00)80772-2
André Nussenzweig, Changhu Chen, Vera da Costa Soares, Mercedes Sanchez, Karen Sokol, Michel C. Nussenzweig, Gloria C. Li, Requirement for Ku80 in growth and immunoglobulin V(D)J recombination Nature. ,vol. 382, pp. 551- 555 ,(1996) , 10.1038/382551A0
Serge Gravel, Michel Larrivee, Pascale Labrecque, Raymund J Wellinger, Yeast Ku as a Regulator of Chromosomal DNA End Structure Science. ,vol. 280, pp. 741- 744 ,(1998) , 10.1126/SCIENCE.280.5364.741
Y. Gu, S. Jin, Y. Gao, D. T. Weaver, F. W. Alt, Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 8076- 8081 ,(1997) , 10.1073/PNAS.94.15.8076
P. Slijepcevic, M. P. Hande, S. D. Bouffler, P. Lansdorp, P. E. Bryant, Telomere length, chromatin structure and chromosome fusigenic potential. Chromosoma. ,vol. 106, pp. 413- 421 ,(1997) , 10.1007/S004120050263
Constance I. Nugent, Giovanni Bosco, Lyle O. Ross, Sara K. Evans, Andrew P. Salinger, J.Kent Moore, James E. Haber, Victoria Lundblad, Telomere maintenance is dependent on activities required for end repair of double-strand breaks Current Biology. ,vol. 8, pp. 657- 662 ,(1998) , 10.1016/S0960-9822(98)70253-2