Novel SNP markers in InvGE and SssI genes are associated with natural variation of sugar contents and frying color in Solanum tuberosum Group Phureja.

摘要: Potato frying color is an agronomic trait influenced by the sugar content of tubers. The candidate gene approach was employed to elucidate molecular basis this in Solanum tuberosum Group Phureja, which mainly diploid and represents important genetic resource for potato breeding. objective research identify novel variants related with quality loci key functions carbohydrate metabolism, purpose discovering variability useful breeding programs. Therefore, association analysis implemented 109 SNP markers identified ten genes. analyses revealed four associations locus InvGE coding apoplastic invertase one SssI a soluble starch synthase. SNPs SssI-C 45711901 T InvGE-C 2475454 were associated sucrose color, respectively, not found previously tetraploid genotypes. rare haplotype InvGE-A 2475187 C 2475295 A 2475344 higher fructose contents. Our study allowed more detailed sequence variation exon 3 from InvGE, possible previous studies because high frequency insertion-deletion polymorphisms potatoes. mapping strategy using Phureja identification tuber measured High Performance Liquid Chromatography (HPLC). These might be programs improving traits increase crop variability. results suggest that some genes involved natural are conserved both germplasm. Nevertheless, types germplasm present different regions these This contributes understanding architecture contents at harvest Phureja.