The impact of various scaffold components on vascularized bone constructs.

摘要: Bone tissue engineering is gaining more interest in the field of craniofacial surgery where continuous efforts are being made to improve outcomes via modulation scaffold components. In an in vitro three dimensional (3D) culture, effect bone morphogenic protein 2 (BMP2, 60 μg/ml) and different cell seeding densities (0.25, 0.5, 1 × 104) rat mesenchymal stem cells seeded on nanocrystalline hydroxyapatite silica gel matrix (Nanobone®) viability differentiation were studied. Alkaline phosphatase assays performed at day 7, 14, 21 assess relative fraction viable 3D cultures. a subsequent in vivo study, we examined axial vascularization, scaffold's particle size nature (collagen type I vs. diluted fibrin) vascularization generation vascularized construct rats. Regarding compared constructs randomly by extrinsic from periphery implanted with others axially arteriovenous loop (AVL). size, having 0.2 mm (powder) other 2 × 0.6 mm (granules). collagen fibrin matrix. Various groups regarding amount generation, cellular proliferation. The initial density had temporary minimal overall osteogenic cells. On contrary, adding BMP2 concentration 60 μg/ml over one week led enhanced despite depressed viability. Axial was mandatory for efficient formation construct. Collagen smaller provided favorable results terms than larger Nanobone particles.