Measurement of glycosylated hemoglobin by immunoassay

摘要: A method for determining glycosylated hemoglobin in a diluted blood sample includes binding of to specific monoclonal antibody and nonglycosylated polyclonal antibody. The binds all fractions blocks the peroxidase activity thereof, except bound which retains activity. beam light having wavelength about 416 nm is passed through assay medium absorbed by fractions, or unbound, this absorbance thereby provides measure total hemoglobin. Hydrogen peroxide substrate are added. oxidized product an at different from nm, oxidation being catalyzed retained This From two absorbances, percentage may be calculated. invention kit materials useful performing invention.