Intercalated Cell Subtypes in Connecting Tubule and Cortical Collecting Duct of Rat and Mouse

摘要: At least two populations of intercalated cells, type A and B, exist in the connecting tubule (CNT), initial collecting (ICT), cortical duct (CCD). Type cells secrete protons via an apical H+-ATPase reabsorb bicarbonate by a band 3-like Cl-/HCO3-exchanger, AE1, located basolateral plasma membrane. B Cl-/HCO3- exchanger that is distinct from AE1 remains to be identified. They express membrane vesicles throughout cytoplasm. third cell with H+-ATPase, but no has been described CNT CCD both rat mouse. The prevalence not known. aim this study was characterize quantify subtypes, including newly non A-non cell, CNT, ICT, triple immunolabeling procedure developed which antibodies 3 protein were used identify subpopulations segment-specific distal segments. In mouse, constituted approximately 40% CCD. A, observed all three segments, being most prevalent species. however, more than half 39% 22% CCD, compared 14, 7, 5%, respectively, rat. contrast, accounted for only 8 16% segments mouse 26 It concluded striking differences distribution different types rat, are fairly rare, whereas they constitute major fraction primarily at expense cells.