Isolation and Characteristics of Brachybacterium sp. SY -97 Killing the Harmful Dinoflagellate Cochlodinium polykrikoides

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DOI: 10.5322/JES.2009.18.4.435

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摘要: A bacterial strain SY-97 that showed algicidal activity against Cochlodinium polykrikoides was isolated from coastal water of Uljin (eastern coast Korea) in August, 2005. The identified as Brachybacterium sp. by morphological and biological tests, analysis 16S rDNA sequence. optimal culture conditions for the growth were 30℃, initial pH 7.0, salinity 2.0%. From result cell insert experiment, is assumed to produce secondary metabolites which have activity. When 10% filtrate this applied C. (1.2 × 104 cells/㎖) cultures, 100% cells destroyed within 15 hours. released algicides heat-tolerant 100℃ stable 6.0~10.0. These results suggest potentially useful controlling outbreaks polykrikoides.

参考文章(21)
Chi-Yong Ahn, Seung-Hyun Joung, Jong-Woon Jeon, Hee-Sik Kim, Byung-Dae Yoon, Hee-Mock Oh, Selective control of cyanobacteria by surfactin-containing culture broth of Bacillus subtilis C1. Biotechnology Letters. ,vol. 25, pp. 1137- 1142 ,(2003) , 10.1023/A:1024508927361
Xiulin Wang, Liangyu Gong, Shengkang Liang, Xiurong Han, Chenjian Zhu, Yanbin Li, None, Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa Harmful Algae. ,vol. 4, pp. 433- 443 ,(2005) , 10.1016/J.HAL.2004.06.001
Sun-Og LEE, Junichi KATO, Katsunori NAKASHIMA, Akio KURODA, Tsukasa IKEDA, Noboru TAKIGUCHI, Hisao OHTAKE, Cloning and Characterization of Extracellular Metal Protease Gene of the Algicidal Marine Bacterium Pseudoalteromonas sp. Strain A28 Bioscience, Biotechnology, and Biochemistry. ,vol. 66, pp. 1366- 1369 ,(2002) , 10.1271/BBB.66.1366
Takuji Nakashima, Yousuke Miyazaki, Yukihiko Matsuyama, Wataru Muraoka, Kenichi Yamaguchi, Tatsuya Oda, Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium Applied Microbiology and Biotechnology. ,vol. 73, pp. 684- 690 ,(2006) , 10.1007/S00253-006-0507-2
Jian Qiang Su, Xiao Ru Yang, Tian Ling Zheng, Yun Tian, Nian Zhi Jiao, Li Zhe Cai, Hua Sheng Hong, Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense Harmful Algae. ,vol. 6, pp. 799- 810 ,(2007) , 10.1016/J.HAL.2007.04.004
JH Skerratt, JP Bowman, G Hallegraeff, S James, PD Nichols, Algicidal bacteria associated with blooms of a toxic dinoflagellate in a temperate Australian estuary Marine Ecology Progress Series. ,vol. 244, pp. 1- 15 ,(2002) , 10.3354/MEPS244001
Young Sik Lee, Sang Yong Lee, Factors affecting outbreaks of Cochlodinium polykrikoides blooms in coastal areas of Korea Marine Pollution Bulletin. ,vol. 52, pp. 626- 634 ,(2006) , 10.1016/J.MARPOLBUL.2005.10.015
Karen G. Porter, Yvette S. Feig, The use of DAPI for identifying and counting aquatic microflora1 Limnology and Oceanography. ,vol. 25, pp. 943- 948 ,(1980) , 10.4319/LO.1980.25.5.0943