APhox2b::FLPotransgenic mouse line suitable for intersectional genetics

摘要: Phox2b is a transcription factor expressed in the central and peripheral neurons that control cardiovascular, respiratory, digestive functions essential for their development. Several populations known or suspected to regulate visceral express developing hindbrain. Extensive cell migration lack of suitable markers have greatly hampered studying Reasoning intersectional fate mapping may help overcome these impediments, we generated BAC transgenic mouse line, P2b::FLPo, which expresses codon-optimized FLP recombinase expressing cells. By partnering P2b::FLPo with FLP-responsive RC::Fela allele, show recombination switches on lineage tracers cells Phox2b, permanently marking them study across Taking advantage dual-recombinase feature RC::Fela, further transgene can be partnered Lbx1Cre as Cre driver generate triple transgenics having history both Lbx1 expression are specifically labeled. Hence, line when provides tool tracking accessing genetically subsets Phox2b-expressing neuronal populations, has not been possible by Cre-mediated alone. genesis 51:506–514. © 2013 Wiley Periodicals, Inc.