Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro.

摘要: Per- and polyfluoroalkyl substances (PFAS) are used as industrial surfactants chemical coatings for household goods such Teflon. Despite regulatory efforts to phase out legacy PFAS, they remain detectable in drinking water throughout the United States. This is due stability of PFAS continued use replacement compounds. In humans, have been detected placenta cord blood associated with low birth weight preeclampsia risk. Preeclampsia a leading cause maternal mortality driven by insufficient endometrial trophoblast invasion, resulting poor placental flow. alter invasion other cell types, but their impact on trophoblasts not understood. We therefore assessed effects migration, gene expression vitro. Trophoblast migration were using modified scratch assay absence or presence Matrigel, respectively. Treatment perfluorooctanoic sulfate (PFOS), acid (PFOA), GenX (1000 ng/ml) each decreased over 24 h. However, only significantly inhibited invasion. PFOS, PFOA, also chemokines (eg, CCL2), chemokine receptors CCR4), inflammatory enzymes ALOX15) involved migration. Inhibition pertussis toxin (10 ng/ml), G-protein inhibitor, similar PFAS. Taken together, decrease signaling. By understanding mechanisms involved, it may be possible identify biological exposure factors that contribute preeclampsia.