The Glutamine-rich Region of the HIV-1 Tat Protein Is Involved in T-cell Apoptosis
作者： Grant R. Campbell , Eddy Pasquier , Jennifer Watkins , Veronique Bourgarel-Rey , Vincent Peyrot
关键词:
摘要: Human immunodeficiency virus (HIV) infection and the progression to AIDS are characterized by depletion of CD4+ T-cells. HIV-1 leads apoptosis uninfected bystander cells direct killing HIV-infected cells. This is mediated, in part, Tat protein, which secreted virally infected taken up We chemically synthesized two 86-residue subtype D proteins, Ug05RP Ug11LTS, from Ugandan patients who were clinically categorized as either rapid progressor or long-term survivor, with non-conservative mutations located essentially glutamine-rich region. Structural heterogeneities revealed CD, translate into differing trans-activational apoptotic effects. CD data analysis molecular modeling indicated that short α-helix observed proteins such Mal not present Ug11LTS. show more efficient than Ug11LTS its role inducing binding tubulin via mitochondrial pathway. The region appears be involved Tat-mediated
sci-hub.se 参考文章(68)
Anne Gatignol, Kuan-Teh Jeang, Tat as a transcriptional activator and a potential therapeutic target for HIV-1. Advances in pharmacology. ,vol. 48, pp. 209- 227 ,(2000) , 10.1016/S1054-3589(00)48007-5
Daniel Zagury, Bernard Bizzini, Anne Sill, Alessandro Gringeri, Jean-François Zagury, William Blattner, Abderrahim Lachgar, Hélène Le Buanec, Robert C. Gallo, Houria Hendel, Miguel Carcagno, Marcelo Criscuolo, Arsène Burny, Jay Rappaport, Malcolm Richardson, Antibodies to the HIV-1 Tat protein correlated with nonprogression to AIDS: a rationale for the use of Tat toxoid as an HIV-1 vaccine. Journal of Human Virology. ,vol. 1, pp. 282- 292 ,(1998)
Steven R. Bartz, Michael Emerman, Human Immunodeficiency Virus Type 1 Tat Induces Apoptosis and Increases Sensitivity to Apoptotic Signals by Up-Regulating FLICE/Caspase-8 Journal of Virology. ,vol. 73, pp. 1956- 1963 ,(1999) , 10.1128/JVI.73.3.1956-1963.1999
D. Morgan, Progression to symptomatic disease in people infected with HIV›1 in rural Uganda: prospective cohort study BMJ. ,vol. 324, pp. 193- 197 ,(2002) , 10.1136/BMJ.324.7331.193
B Ensoli, L Buonaguro, G Barillari, V Fiorelli, R Gendelman, R A Morgan, P Wingfield, R C Gallo, Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation. Journal of Virology. ,vol. 67, pp. 277- 287 ,(1993) , 10.1128/JVI.67.1.277-287.1993
Thomas W. McCloskey, Melanie Ott, Savita Pahwa, Eric Verdin, Morris O. Paul, Narendra Chirmule, Narendra Chirmule, Narendra Chirmule, Shabbir A. Khan, Saul Teichberg, Eugene Tribble, Dual role of HIV Tat in regulation of apoptosis in T cells. Journal of Immunology. ,vol. 158, pp. 1014- 1019 ,(1997)
Dietlinde Wolf, Vanessa Witte, Bernd Laffert, Katja Blume, Elisabeth Stromer, Susanna Trapp, Paola d'Aloja, Annette Schürmann, Andreas S. Baur, HIV-1 Nef associated PAK and PI3-kinases stimulate Akt-independent Bad-phosphorylation to induce anti-apoptotic signals. Nature Medicine. ,vol. 7, pp. 1217- 1224 ,(2001) , 10.1038/NM1101-1217
W. Curtis Johnson, Circular Dichroism and Its Empirical Application to Biopolymers Methods of Biochemical Analysis. ,vol. 31, pp. 61- 163 ,(1985) , 10.1002/9780470110522.CH2
James C. Lee, Ronald P. Frigon, Serge N. Timasheff, The chemical characterization of calf brain microtubule protein subunits Journal of Biological Chemistry. ,vol. 248, pp. 7253- 7262 ,(1973) , 10.1016/S0021-9258(19)43386-3
Pascale Barbier, Vincent Peyrot, Marcel Sarrazin, Gregory A. Rener, Claudette Briand, Differential effects of ethyl 5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3,4-b]pyrazin-7-yl carbamate analogs modified at position C2 on tubulin polymerization, binding, and conformational changes. Biochemistry. ,vol. 34, pp. 16821- 16829 ,(1995) , 10.1021/BI00051A033