Clinical Diagnosis of Hepatitis B Infection: Applications of the Polymerase Chain Reaction

作者: Geoffrey Dusheiko , Jianye Xu , Arie J. Zuckermann

DOI: 10.1007/978-3-642-84766-0_7

关键词:

摘要: Hepatitis B virus (HBV) infection serves as a good example of the use polymerase chain reaction (PCR) for diagnosis viral infection. The technique is at least 104 times more sensitive than current dot blot hybridization assays HBV DNA. PCR overcomes disadvantage low concentrations DNA in patients with level viraemia, and permits detection, cloning, sequencing genomes from such patients. Use this assay indicates that loss HBeAg associated decrease rather complete disappearance

参考文章(66)
Michael A. Gerber, Massimo Colombo, James S. Malter, Pietro Lampertico, Detection of hepatitis B virus DNA in formalin-fixed, paraffin-embedded liver tissue by the polymerase chain reaction. American Journal of Pathology. ,vol. 137, pp. 253- 258 ,(1990)
Christian Bréchot, Polymerase chain reaction: A new tool for the study of viral infections in hepatology Journal of Hepatology. ,vol. 11, pp. 124- 129 ,(1990) , 10.1016/0168-8278(90)90282-V
Jay H. Hoofnagle, Jules Dienstag, Girish N. Vyas, Viral Hepatitis and Liver Disease ,(1985)
Peter Bannasch, G. Weber, D. Keppler, Liver cell carcinoma Kluwer Academic. ,(1989)
J. L. Hughes, J. E. Jones, Alan McLachlan, G. B. Thornton, D. R. Milich, R. Houghten, Distinction between immunogenicity and tolerogenicity among HBcAg T cell determinants. Influence of peptide-MHC interaction. Journal of Immunology. ,vol. 143, pp. 3148- 3156 ,(1989)
Rainer Müller, Gundolf Gubernatis, Margarete Farle, Gabriele Niehoff, Heide Klein, Christian Wittekind, Günter Tusch, Hans-Ulrich Lautz, Klaus Böker, Walter Stangel, Rudolf Pichlmayr, Liver transplantation in HBs antigen (HBsAg) carriers Journal of Hepatology. ,vol. 13, pp. 90- 96 ,(1991) , 10.1016/0168-8278(91)90869-D
Osamu Yokosuka, Masao Omata, Kazuhiko Hosoda, Minoru Tada, Toshiki Ehata, Masao Ohto, None, Detection and direct sequencing of hepatitis B virus genome by DNA amplification method Gastroenterology. ,vol. 100, pp. 175- 181 ,(1991) , 10.1016/0016-5085(91)90598-F
C Chang, G Enders, R Sprengel, N Peters, H E Varmus, D Ganem, Expression of the precore region of an avian hepatitis B virus is not required for viral replication. Journal of Virology. ,vol. 61, pp. 3322- 3325 ,(1987) , 10.1128/JVI.61.10.3322-3325.1987
Patrick Marcellin, Emile Giostra, Michele Martinot-Peignoux, Marie-Anne Loriot, Marie-Lorraine Jaegle, Philippe Wolf, Claude Degott, Francoise Degos, Jean-Pierre Benhamou, Redevelopment of Hepatitis B Surface Antigen After Renal Transplantation Gastroenterology. ,vol. 100, pp. 1432- 1434 ,(1991) , 10.1016/0016-5085(91)90799-Q