Modifying ligand specificity of gene regulatory proteins
作者： Donald F Doyle , David J Mangelsdorf , David R Corey
DOI: 10.1016/S1367-5931(99)00052-6
关键词:
摘要: Nuclear receptors contain a conserved hydrophobic ligand binding pocket that is particularly amenable to structure-based protein engineering. Thus, site-directed mutagenesis of the has resulted in creation nuclear with novel specificities. Such proteins are now being used control gene expression vivo ligand-dependent manner.
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