Effect of Pollination Timing on the Rate of Apomictic Reproduction Revealed by RAPD Markers in Paspalum notatum

摘要: Progeny tests employing molecular markers allow the identification of individuals originated by sexual means among offspring a facultative apomict. The objective this work was to evaluate effect pollination timing on proportion sexually formed in progenies apomictic Paspalum notatum genotype. families approx. 30 plants each were generated at five different times: 1–3 d pre‐anthesis; anthesis; and 2, 4 6 d post‐anthesis. Cytoembryological analyses indicated that 17 % ovules carried meiotic cytologically reduced embryo sac florets simultaneously with those used for crosses. parental F1 analysed using RAPD markers. Ninety‐five oligonucleotides assayed progenitors order search male‐specific bands. Eight primers presenting clear polymorphic bands selected use progeny tests. produced reached 3·4 % before anthesis 20 % anthesis, while after only maternal plants. A second 97 plants obtained from 16 off‐type (16·5 %), which one BIII hybrid (2n + n). Our results indicate allows greatest potential sexuality be expressed When is delayed as soon 2 d aposporous sacs develop endosperm through pseudogamy set seed.