Evaluating the efficacy of DNA repair biomarkers to assess human cell response to chemotherapy using imaging flow cytometry

摘要: Chemotherapy and radiotherapy are widely accepted as common forms of treatment for cancers. The majority cancer patients receive chemotherapy alone or in combination with radiotherapy. Most chemotherapeutic drugs cause DNA damage to the rapidly dividing cells but normal also damaged process. Therefore repair levels tumour may determine success treatment. aim this work was evaluate use biomarkers assessing responses drugs. novel technique imaging flow cytometry employed analyse induction resolution γ-H2AX RAD51 cell lines MRC5-SV1 NB1-HTERT, an ATM-deficient line AT5BIVA (derived from Ataxia Telangiectasia patient) XPF-deficient GM08437B. Two were developed, NB1-HTERT which had been made resistant HN2. A range drugs, Adriamycin, Cisplatin Nitrogen Mustard have different modes action examined work. We demonstrated distinct differences foci between two following exposure Additionally, it that both sensitive elevated expression profiles comparison parental over a 48 hour period post cross-linking agent It is concluded while Rad51 be useful determining response, larger cohort samples required further analysis.