Chromogranin A expression in neoplastic cells affects tumor growth and morphogenesis in mouse models.
作者： Antonella Monno , Flavio Curnis , Gianluigi Arrigoni , Chiara Foglieni , Barbara Colombo
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摘要: Chromogranin A (CgA), a secretory protein expressed by many neuroendocrine cells, has been recognized as useful tissue and serum marker of tumors. To investigate the effect CgA secretion on neoplastic morphogenesis progression, we have transfected mouse RMA lymphoma TS/A adenocarcinoma cells with cDNA encoding human selected several CgA-positive (secreting) CgA-negative (nonsecreting) clones. In both models, growth rate clones implanted s.c. in nude mice was slower than that Histological analysis each tumor showed CgA-expression associated multinodular patterns, whereas tumors appeared more compact similar to wild-type Moreover, production increased necrosis. The number nodules correlated levels (n = 40, r 0.537, P 0.0004). reduced not related vitro proliferation or changes cell adhesion shape, suggesting mechanism is indirect host-mediated. These results suggest abnormal could affect morphogenesis.
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Noriko Kimura, Chromogranins in non-endocrine tumours. Advances in Experimental Medicine and Biology. ,vol. 482, pp. 369- 373 ,(2002) , 10.1007/0-306-46837-9_30
B. Helpap, J. Köllermann, Immunohistochemical analysis of the proliferative activity of neuroendocrine tumors from various organs Virchows Archiv. ,vol. 438, pp. 86- 91 ,(2001) , 10.1007/S004280000337
U.M. Benedum, P.A. Baeuerle, D.S. Konecki, R. Frank, J. Powell, J. Mallet, W.B. Huttner, The primary structure of bovine chromogranin A: a representative of a class of acidic secretory proteins common to a variety of peptidergic cells. The EMBO Journal. ,vol. 5, pp. 1495- 1502 ,(1986) , 10.1002/J.1460-2075.1986.TB04388.X
Harlan I. Firminger, Atlas of tumor pathology Armed Forces Institute of Pathology. ,(1968)
H.J. Wu, D.J. Rozansky, R.J. Parmer, B.M. Gill, D.T. O'Connor, Structure and function of the chromogranin A gene. Clues to evolution and tissue-specific expression. Journal of Biological Chemistry. ,vol. 266, pp. 13130- 13134 ,(1991) , 10.1016/S0021-9258(18)98814-9
D S Konecki, U M Benedum, H H Gerdes, W B Huttner, The primary structure of human chromogranin A and pancreastatin. Journal of Biological Chemistry. ,vol. 262, pp. 17026- 17030 ,(1987) , 10.1016/S0021-9258(18)45486-5
Paolo Dellabona, Giulia Casorati, Giulia Fulci, Monica Moro, Angelo Corti, Giovanni Paganelli, Antonio G. Siccardi, Micaela Pelagi, Tumor Cell Targeting with Antibody-Avidin Complexes and Biotinylated Tumor Necrosis Factor α Cancer Research. ,vol. 57, pp. 1922- 1928 ,(1997)
L J Helman, T G Ahn, M A Levine, A Allison, P S Cohen, M J Cooper, D V Cohn, M A Israel, Molecular cloning and primary structure of human chromogranin A (secretory protein I) cDNA. Journal of Biological Chemistry. ,vol. 263, pp. 11559- 11563 ,(1988) , 10.1016/S0021-9258(18)37995-X
Angelo Corti, Lincidio Perez Sanchez, Anna Gasparri, Flavio Curnis, Renato Longhi, Anna Brandazza, Antonio G. Siccardi, Alessandro Sidoli, Production and structure characterisation of recombinant chromogranin A N-terminal fragments (vasostatins) Evidence of dimer-monomer equilibria FEBS Journal. ,vol. 248, pp. 692- 699 ,(1997) , 10.1111/J.1432-1033.1997.00692.X
J P Simon, D Aunis, Biochemistry of the chromogranin A protein family. Biochemical Journal. ,vol. 262, pp. 1- 13 ,(1989) , 10.1042/BJ2620001