Developmental competence of mammalian oocytes-insights into molecular research and the promise of microfluidic technology: a review

摘要: Developmental competence of female gametes determines their maturation ability, successful fertilisa - tion, and proper zygote formation. Oocyte quality may be assessed by expression profiling several gene markers such as Cx43, TGFB, GDF9, BMP, Lox Pdia5 that determine the biological features oocytes. Conversely, other extrinsic factors, including follicular size or heat shock significantly influence oocyte ability to grow develop during folliculo- oogenesis. However, using molecular methods for evaluation often leads destruction an analysed cell. Therefore, there is increased requirement seek new non-invasive oocyte-embryo assessment. Here we describe Lab-on-Chip system based on microfluidic technology, which first parametric objective device developmental spectral images. In this review factors are discussed. Furthermore, our previous studies, discuss possibility applying spectrophotometric (Lab-on-Chip) in both biomedical reproductive research domestic animals.