Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells.

摘要: Local mechanical properties were measured for bovine endothelial cells exposed to shear stress using an atomic force microscopy (AFM), and the AFM indentations simulated a finite element method (FEM) determine elastic modulus. After exposure stress, showed marked elongation orientation in flow direction, together with significant decrease peak cell height. The applied force-indentation depth curve was obtained at seven different locations on major axis of surface quantitatively expressed by quadratic equation. modulus determined comparison experimental numerical results. our FEM model significantly became higher from 12.2+/-4.2 18.7+/-5.7 kPa stress. Fluorescent images that fibers F-actin bundles mainly formed central portion sheared cells. increase may be due this remodeling cytoskeletal structure. moduli Hertz are 0.87+/-0.23 1.75+/-0.43 control respectively. This difference can attributable differences approximation functions would contribute better understanding local