Synthesis and characterization of multifunctional hybrid-polymeric nanoparticles for drug delivery and multimodal imaging of cancer.

摘要: In this study, multifunctional hybrid-polymeric nanoparticles were prepared for the treatment of cultured multicellular tumor spheroids (MCTS) PANC-1 and MIA PaCa-2 pancreatic carcinoma cell lines. To synthesize nanoparticles, poly lactic-co-glycolic acid core particles was loaded with Rhodamine 6G dye chemotherapeutic agent, Paclitaxel, incorporated into outer phospholipid layer. The surface coated gadolinium chelates magnetic resonance imaging applications. This engineered nanoparticle formulation found to be suitable use in guided therapy. Specifically, we investigated size-dependent therapeutic response uptake that 65 nm, 85 110 nm size MCTS two cancer lines used. After 24 hours treatment, both showed an average increase 18.4% 24.8% nanoparticles. Furthermore, studies on effects particle had a slight influence overall effectiveness formulation. line, produce greatest effect, whereas 12.8% cells apoptotic which 11.4% 9.79% Finally, study conducted vivo revealed importance selection effective delivery drug formulations tumors. agreement our vitro results, excellent retention tumors tumor-bearing mice treated