Efficient hydrophilic interaction liquid chromatography–tandem mass spectrometry for the multiclass analysis of veterinary drugs in chicken muscle

摘要: Abstract A simple and sensitive method has been developed for multiresidue analysis of 24 important veterinary drugs (including 3 aminoglycosides, β-lactams, 2 lincosamides, 4 macrolides, quinolones, sulfonamides, tetracyclines, amprolium) in chicken muscle. The involved a extraction using (1:1, v/v) 2% trichloroacetic acid water–acetonitrile, followed by removing fat with hexane, dilution sample extract, filtration prior to liquid chromatography–tandem mass spectrometric (LC–MS/MS) analysis. Hydrophilic interaction chromatography (HILIC) proved be very effective separation wide range polar hydrophilic compounds (providing high sensitivity good peak shape) compared reversed phase ion-pair separation. was successfully validated according the European Decision 2002/657/EC. Average recoveries were 53–99% at 0.5-MRL, MRL, 1.5-MRL spiking levels, satisfactory precision ≤15% RSD. limit detection 0.1–10 μg kg −1 22 analytes 20 μg kg aminoglycosides. These values lower than maximum residue limits (MRLs) established Union. evaluated provides reliable screening, quantification, identification drug residues foods animal origin. It tested real samples (such as muscle, shrimp, egg).