Parental Genome Separation in Reconstructions of Somatic and Premeiotic Metaphases of Hordeum Vulgare × H. Bulbosum

摘要: A stable interspecific sexual plant hybrid between Hordeum vulgare cv. Tuleen 346 (barley) × H. bulbosum was shown to have seven chromosomes originating from each parent by genomic in situ hybridization. Electron microscope serial thin-section reconstructions of metaphases and comparison with light micrograph karyo-types enabled be identified their morphology. The three-dimensional positions centromeres were established analysed the somatic root tip cells at mitotic metaphase near entry into meiosis. Parental genomes tended lie spatially separated domains both tissues. Although varying morphology, two sets had similar mean sizes, so size differences did not cause separation observed. In EM, centromere-associated structures more central genome, , larger than those peripheral genome origin.