Development of DNA Damage Response Signaling Biomarkers using Automated, Quantitative Image Analysis

摘要: The DNA damage response (DDR) coordinates repair with cell cycle checkpoints to ameliorate or mitigate the pathological effects of damage. Automated quantitative analysis (AQUA) and Tissue Studio are commercial technologies that use digitized immunofluorescence microscopy images quantify antigen expression in defined tissue compartments. Because DDR is commonly activated cancer may reflect genetic instability within lesion, a method offers potential diagnostic and/or prognostic value. In this study, both AQUA algorithms were used radiation-damaged skin fibroblasts, melanoma lines, moles, primary metastatic melanomas. Digital image results for three markers (γH2AX, P-ATM, P-Chk2) correlated immunoblot data irradiated whereas only γH2AX P-Chk2 lines. Melanoma lines displayed substantial variation expression, was significantly radioresistance. Moles, melanomas, metastases brain, lung liver similar observed digital immunofluorescent stained biomarkers be useful predicting tumor radiation chemotherapy.