Acidic “peptidophospholipids”, a new class of hapten-bearing cell surface modifying reagents

摘要: Abstract Covalent coupling of glutamyl-glutamic acid to the amino group ether-phosphatidylethanolamine (EPE) yields an acidic “peptidophospholipid” (Glu 2 -EPE) which is water-soluble above pH 7.0 and stable phospholipase A. The terminal Glu -EPE free for with amino-reactive determinants. We describe synthesis various hapten-substituted peptidophospholipids as well intermediate compound, coupled heterobifunctional reagent 3-(2'-pyridyl)-dithiopropionic N -hydroxysuccinimide ester. latter derivative allows binding sulfhydryl-containing molecules, e.g. peptides or proteins. So far, beef pig insulin trinitrophenyl-substituted ribonuclease A have thus been linked -EPE. All derivatives ofGlu are at physiological readily adsorb cell surfaces from aq. solution. Binding cells fast, “non-toxic” over a wide range concns. adsorbed determinants accessible specific antibodies facilitate complement-mediated lysis. appears be universal carrier molecule simple mild modification foreign determinants, Jerne plaque assays.