Radiation-induced phenotypic alterations in relation to isozymes and RAPD markers in Vigna radiata (L.) Wilczek
作者： Sumana Roy , Yasmin Begum , Anindita Chakraborty , Sarmistha Sen Raychaudhuri
DOI: 10.1080/09553000600969804
关键词:
摘要: Purpose: The present investigation is aimed at studies on the effects of gamma rays in vitro and vivo damage Vigna radiata. The parameters studied are germination frequency, seedling injury, isozyme alteration random amplified polymorphic DNA (RAPD) markers. Results obtained analyzed light modern applications radiation damage.Materials methods: Seeds radiata were subjected to irradiation with a dose 20 – 200 Gy. percent frequency determined. Callus samples produced exposed rays. irradiated callus processed extract total protein, specifically stained for superoxide dismutase (SOD) peroxidase isozymes. Total genomic was extracted from analysis using 23 decamer primers.Results: Gamma resulted retardation height decrease freque...
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