Molecular epidemiology of clinical and carrier strains of methicillin resistant Staphylococcus aureus (MRSA) in the hospital settings of north India

摘要: The study was conducted between 2000 and 2003 on 750 human subjects, yielding 850 strains of staphylococci from clinical specimens (575), nasal cultures hospitalized patients (100) eye & sources hospital workers (50 125 respectively) in order to determine their epidemiology, acquisition dissemination resistance genes. Organisms samples were isolated, cultured identified as per the standard routine procedures. Susceptibility measured by agar diffusion method, recommended Nat ional Committee for Clinical Laboratory Standards (NCCLS). modified method Birnboin Takahashi used isolation plasmids staphylococci. Pulsed-field gel electrophoresis (PFGE) typing carrier Methicillin resistant Staphylococcus aureus (MRSA) isolated during our performed described previously. It shown that 35.1% 22.5% coagulase-negative staphylococcal isolates methicillin. Highest percentage MRSA (35.5%) found pus (n = 151). multiple drug all 180) Coagulase-negative (MRCNS) 76) detected. In case both methicillin-resistant well methicillin-sensitive Saphylococcal zero vancomycin where highest penicillin G followed ampicillin. major reservoir methicillin hospitals are colonized/infected inpatients colonized workers, with carriers at risk developing endogenous infection or transmitting health care patients. results confirmed molecular using PFGE Sma I-digestion. markers T got transferred S. (JS-105) (JN-49) ciprofloxacin (Cf) erythromycin (E) seemed be chromosomal mediated. one experiments, plasmid pJMR1O coding ampicillin (A), gentamicin (G) amikacin (Ak) transformed into Escherichia coli. minimal inhibitory concentrations (MICs) A lower E. coli than aureus. However, MIC Ak higher transformants There is a progressive increase prevalence multi-drug Vancomycin still choice infections. workers. Resistance transfer due antibiotic stress an alarming threat antimicrobial chemotherapy.