Intracerebral microdialysis of adenosine and adenosine monophosphate - a systematic review and meta-regression analysis of baseline concentrations

摘要: Microdialysis is a method to study the extracellular space in vivo, based on principle of diffusion. It can be used measure various small molecules including neuroregulator adenosine. Baseline levels compounds measured with microdialysis vary over studies. We systematically reviewed literature investigate full range reported baseline concentrations adenosine and monophosphate microdialysates. performed meta-regression analysis influence flow rate, probe membrane surface area, species, brain area anaesthesia versus freely behaving, concentration. microdialysates ranged from 0.8 2100 nM. There was limited evidence Across studies, we found effects rate behaving dialysate (p ≤ 0.001), but not surface, or ≥ 0.14). With increasing decreased. anaesthesia, increased. The effect other predictor variables concentrations, for example, post-surgical recovery time, could analysed because lack data. This shows that as an alternative new animal experiments answer research questions field neurochemistry. However, current reporting primary studies are insufficient reach potential this approach; 63 out 133 included reporting, several potentially relevant factors had excluded analyses. level experimental detail needs improve.